The League of Shadows are revealed to have manipulated the Court of Owls into doing their bidding. Some of it's assassins attacked Fish Mooney, Oswald Cobblepot, Hugo Strange, Victor Fries, and Bridgit Pike at a slaughterhouse where Hugo Strange's earlier cures for Alice Tetch's poisonous blood was being held.
Most of the assassins there were killed by James Gordon which also ended with Fish Mooney accidentally getting killed. Other assassins from the League of Shadows were present at the Yuyan Building when Bruce Wayne first meets Ra's al Ghul.
- Hugo Strange (formerly)
- The Court of Owls (manipulated)
- Bruce Wayne (manipulated)
- In the comics, the League of Assassins (which has also been called the League of Shadows in different media appearances) is an organization that is led by Ra's al Ghul and have their own plans to preserve the world their way.
- The League of Assassins bear striking resemblances to the Hashshashin, a Medieval Levantine Order of trained combatants and assassins, who fought during the Crusades and were also lead by a mysterious and enigmatic mentor called the "Old Man of the Mountain".