The League of Shadows are revealed to have manipulated the Court of Owls into doing their bidding. Some of it's ninjas attacked Fish Mooney, Oswald Cobblepot, Hugo Strange, Victor Fries, and Bridgit Pike at a slaughterhouse where Hugo Strange's earlier cures for Alice Tetch's poisonous blood was being held. Most of the assassins there were killed by James Gordon which also ended with Fish Mooney accidentally getting killed. Other assassins from the League of Shadows were present at the Yuyan Building when Bruce Wayne first meets Ra's al Ghul.
After killing Niles Winthrop which led to Alex Winthrop going on the run, Ra's al Ghul meets with Barbara Kean as he sends Anubis and The Hunter to track Alex down. While Bruce and Jim Gordon defeat the two operatives, Ra's al Ghul shows up and holds Alex at knifepoint in order to get the embalming knife from them. When Bruce was reluctant to give up the embalming knife, Ra's al Ghul slit Alex's throat and then surrendered to Gordon.
To further his plans, Ra's al Ghul secretly had some members of the League of Shadows infiltrate Blackgate Penitentiary and replace some of the guards. When Barbara Kean visits him with a plan to break him out, Ra's al Ghul declines her offer and transferred some of his energy to Barbara for her to use at a later date. When Bruce Wayne infiltrates Blackgate Penitentiary to deal with Ra's al Ghul, he is subdued by the disguised League of Shadows members and taken to Blackgate Penitentiary's sub-basement. While the fake prison guards deal with Jim Gordon and Alfred Pennyworth, Ra's al Ghul tempts Bruce into using the embalming knife on him to end his curse. Jim Gordon and Alfred Pennyworth defeat the guards and arrive in the sub-basement to find that Bruce had given in to Ra's al Ghul's temptation and had used the embalming knife on him. When Blackgate Penitentiary goes into lockdown after what happened inside, Alfred talks to the police while Gordon tells Bruce that they will cover up what happened by telling the public that the League of Shadows infiltrated Blackgate Penitentiary and made off with Ra's al Ghul.
- Barbara Kean (associate, formerly)
- Anubis (tracker, deceased)
- The Hunter (hunter, deceased)
- Hugo Strange (associate of Sensei, formerly)
- The Court of Owls (manipulated)
- Bruce Wayne (manipulated)
- "A Dark Knight: Pax Penguina"
- "A Dark Knight: The Fear Reaper"
- "A Dark Knight: They Who Hide Behind Masks"
- "A Dark Knight: The Demon's Head"
- "A Dark Knight: The Blade's Path"
- In the comics, the League of Assassins (which has also been called the League of Shadows in different media appearances) is an organization that is led by Ra's al Ghul and have their own plans to preserve the world their way.
- The League of Assassins bears striking resemblance to the Hashshashin, a Medieval Levantine Order of trained combatants and assassins who fought during the Crusades and were also lead by a mysterious and enigmatic mentor called the "Old Man of the Mountain".
- ↑ Cannon, Danny (writer) & Hope, Nathan (director) (June 5, 2017) "Heroes Rise: Destiny Calling" Gotham. Season 3. Episode 21. FOX.
- ↑ Lilien, Steven & Wynbrandt, Bryan (writer) & Tonderai, Mark (director) (October 5, 2017). "A Dark Knight: They Who Hide Behind Masks". Gotham. Season 4. Episode 3. FOX.
- ↑ McKenzie, Ben (writer) & Fink, Kenneth (director) (October 13, 2017). "A Dark Knight: The Demon's Head". Gotham. Season 4. Episode 4. FOX.
- ↑ Chun, Tze (writer) & White, Scott (director) (October 19, 2017) "A Dark Knight: The Blade's Path". Gotham. Season 4. Episode 5. FOX.